Preliminary exploration on operation process for autologous ozonized blood transfusion / 中国输血杂志

Autologous ozonized blood transfusion(AOBT) is a therapy of re-transfusion of 100-200 mL of autologous blood after shaking and agitation with appropriate amount of oxygen-ozone in vitro. The oxidation of blood through the strong oxidation of ozone can enhance the non-specific immune response of the body, regulate the internal environment and promote health. This therapy has been increasingly applied in clinical practice, while no unified standard for the operation process in terms of ozone concentration, treatment frequency and treatment course had been established. This operation process of AOBT is primarily explored in order to standardize the operation process and ensure its safety and efficacy.

Study of the effects of three different types of transportations on the quality of red cell suspension / 中国输血杂志

Objective To study the effects of aircraft, railway and highway transportations on the quality of red cell suspension.Methods Red cell suspensions from aircraft, railway and highway transportations were randomly selected, 10 units each, and stored for about 20 days as test groups, Another 10 unites of red cell suspensions were collected and stored for 20 days to serve as the control group. Biochemical indicators, including lactic dehydrogenase(LDH), Na+, K+ and Cl-were tested by automatic biochemistry analyzer. Free hemoglobin (FHb) was measured by chromatometry of 3 wavelengths. PO2 was detected by blood gas analyzer. The rate of red blood anaerobic glycolysis and osmotic fragility were also tested. The microstructure of red blood cells (RBCs) was observed by scanning electron microscope.Results The rates of anaerobic glycolysis of aircraft and railway transported red cell suspensions were (42.9?5.6)% and (42.2?3.1)%, respectively, significantly lower than those of highway transportation and the control group, which were (60.4?5.9)% and (70.1?7.8)%, respectively. FHb concentrations in erythrocyte suspension from aircraft and railway transportations were (1.37?0.31) mg/ml and (1.28?0.41) mg/ml, respectively, significantly higher than those of the highway transportation and the control group, which were (0.37?0.05) mg/ml and (0.31?0.03) mg/ml, respectively. LDH level and Na+ concentrations in erythrocyte suspension from aircraft and railway transportations were significantly higher than those from highway transportation and the control group. PO2 was decreased significantly in red cell suspension from aircraft transportation, and the osmotic fragility was increased. K+ was increased significantly in red cell suspension from railway transportation. Cl-was not changed. The aircraft and railway transported RBCs showed agglutination, spherical changes and ragged edges. Many thorn RBCs could be found in these samples. The morphology of RBCs after highway transportation had normal shape , but a few were spherical and ragged at edges. The RBCs of the control group was in double-faced concave disc shape and equably suspended.Conclusion The quality of red cell suspension is closely related to the different ways of transportation. Under certain circumstances, the quality of red cell suspension from highway transportation is superior to that from aircraft and railway transportations.

Test and comparison of some factors in platelet-cryoprecipitate suspension / 第三军医大学学报

0.05) from that of the cryoprecipitate, but was shorter than that in the FFP and frozen platelet. Conclusion The clinical use of the platelet cryoprecipitate suspension is superior to that of common blood components, because it includes platelets and coagulable factors.